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Abstract
A quantitative method for the determination of intact saponins in Lupinus angustifolius seed by high-performance liquid chromatography (HPLC) is described. The seed was extracted under mild conditions with 70% aqueous ethanol containing 0.01% EDTA and quantified using an internal standard of α-hederin. Soyasaponin VI, also known as soyasaponin βg, a DDMP (2,3-dihydro—2,5-dihydroxy—6-methyl—4H-pyran—4-one)- conjugated form of soyasaponin I, was the only saponin detected in the lupin seed. The technique is compared with a gas chromatographic method which quantifies the sapogenol resulting from the acid hydrolysis of the saponins.