Abstract
An application of high performance liquid chromatography (HPLC) with electrochemical detection to investigate the hydroxyl radical production in the testes of cadmium-treated mice is described. Salicylate was used as a free radical trapping agent to trap hydroxyl radicals in vivo in the testes of cadmium-treated mice. Using this HPLC method, the products formed by hydroxyl radical addition to salicylate, 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-dihyroxybenzoic acid (2,5-DHBA), were separated and quantitated. It was found that the concentrations of both 2,3-DHBA and 2,5-DHBA in the testes of cadmium-treated mice were significantly higher than that without the treatment of cadmium. This study demonstrated that the tissue damage induced by cadmium was the result of the increase in the production of hydroxyl radicals.