Direct Chromatographic Separation of 2-(2-Amino-1, 3-thiazol-4-yl)methylglycine and Its Methyl Ester Enantiomers Using a Chiral Crown Ether Column

Abstract

Direct liquid chromatographic separation of 2-(2-amino-1, 3-thiazol-4-yl) methylglycine and its methyl ester enantiomers was achieved using a chiral crown ether column. The separation was strongly influenced by column temperature and the pH of mobile phase. The method can detect the undesired (+)-enantiomer down to a level of 0.5% and is routinely employed to determine the purity of the desired (−)-enantiomer.