Endophytic Serratia sp. PW7 shifts bacterial endophytes in wheat (Triticum aestivum L.) to reduce pyrene contamination

Abstract

Endophytes in plants are an essential part of plant and participate in the functioning of plants. In this study, the endophytic bacterial communities in the inoculated wheat seedlings were accessed to reveal the effects of functional endophytic bacterium inoculation. The results showed that inoculation with endophytic strain Serratia sp. PW7 shifted endophytic bacterial communities and promoted bacterial diversities in inoculated plant, accompanied with the promotion of pyrene removal and plant growth. Pyrene exposure was beneficial for Serratia sp. PW7 growing and reproducing in the inoculated seedlings, and the endophytic bacterium alteration and pyrene removal were significantly positively correlated with the colonization efficiency of Serratia sp. PW7. The inoculation efficiency was high in those seedlings inoculated via roots and seeds. Inoculation had significant positive effects on the bacterial community diversities and no significant effects on the bacterial richness. More promotion of Shannon indices was observed on bacterial diversity in the inoculated seedlings grown under high pyrene contamination. The Shannon indices of endophytic bacteria in shoots increased by 23.1–37.2% as exposed to 100 μg·L⁻¹ pyrene, and by 70.8–112.5% as exposed to 500 μg·L⁻¹ pyrene.

Keywords:

• Bacterial diversity
• functional endophytic bacterium
• inoculation
• pyrene
• Serratia sp.

Disclosure statement

All authors have no conflict of interest to declare.

Authors contribution

Shuang Li and Xuezhu Zhu designed the experiments, analyzed the data and wrote the article; Shuang Li and Haiyan Zhao performed the cultivation experiments; Danqin Wang analyzed the biomass and pyrene; Xue Wang analyzed the diversity of endophytic bacteria. All authors evaluated and revised the manuscript writing.

Additional information

Funding

This work was financially supported by the National Key R & D Plan Key Project (2019YFC1804301) and National Natural Science Foundation of China (31670514).