The Molecular Ecology Analysis of Microbial Communities in Waste Water–based Mud

Abstract

The molecular ecology analysis of microorganisms is important for the development of biological treatment of waste water–based mud. The bacterial 16S rRNA gene clone library was constructed to detect the community diversity in waste drilling fluid in this study. The phylogenetic analysis indicated that most of clones were clustered in β, γ-proteobacteria with the phylotypes belonging to γ-proteobacteria accounting for 78.6% of the clone library. Halomonas sp. and Chromohalobacter sp. as moderately halophilic bacteria were the main bacteria in waste water–based mud, accounting for 47.8% of the total of clones. This study provides the certainty of chance of the improvement of biological treatment by the selection of dominant strain.

Keywords:

• 16S rRNA
• diversity of microbial communities
• PCR
• phylogenetic analysis
• waste water–based mud