Abstract
Biosurfactants as a group of secondary metabolites are produced by microorganisms. These molecules reduce surface tension in water and hydrocarbon mixtures. To produce biosurfactants, two different media with different concentrations of carbon and nitrogen sources were used. The largest production rate of biosurfactants occurred at 30°C with the volumes of 2 and 3 mL for vegetable oil and 3 mL for crude oil and concentration of 3 mg/mL for nitrogen source. According to the results of this study, establishing optimal conditions has an effective role in the production of biosurfactants and seems to be an appropriate solution for increasing production.