Abstract
In this study, analysis methods for the PAH metabolites of naphthalene (Na), pyrene (Py) and benzo(a)pyrene (BaP) with different benzo-rings (2-4-5 rings respectively) were developed and the metabolism kinetics of Py and BaP in marine fishes were studied. Two PAH metabolites of Na and Py, namely 1-naphthol (1-OH Na) and 1-hydroxy pyrene (1-OH Py), were determined using the fixed wavelength fluorescence (FF) method, and the BaP metabolite, 3-hydroxy benzo(a)pyrene (3-OH BaP), was determined using reverse-phase HPLC with fluorescence detection. The dose- and time-response of Lateolabrax japonicus to Py metabolites and Sparus macrocephalus to BaP metabolites were studied in order to evaluate the use of PAH metabolites as a means of assessing exposure to PAHs. The results showed that both fishes could be induced to metabolize and eliminate their metabolites in vivo with increasing Py and BaP exposure concentrations in seawater. As Py and BaP concentrations increased, metabolite concentrations in the fish bile also increased. A significant dose-response of biliary PAH metabolites was observed after exposure for 1, 3 and 7 days for Py and 2, 4 and 7 days for BaP, respectively. These results provide the proof necessary for using PAH metabolites in marine fishes as a specific biomarker or early warning signal of PAH pollution in the marine coastal environment.
Acknowledgments
This work was supported by Natural Science Foundation of China grants (No. 20077023, 40106012, 40476048). We thank Professor I.J. Hodgkiss of The University of Hong Kong for help with the English.
This paper was presented at the SETAC Asia/Pacific 2006 conference held at Peking University, China on September 18-20, 2006. Received July 31, 2007.