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Original Articles

The interference of scopoletin with measurement of histamine content of cotton dust

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Pages 289-296 | Accepted 23 Dec 1980, Published online: 15 Dec 2008
 

Abstract

Scopoletin (7‐hydroxy‐6‐methoxycoumarin) is a major extractable component of cotton plant parts. It exhibits a strong blue fluorescence peak when activated in the near uv region. Further, histamine has been reported to be present in cotton plant parts. The results reported here show that scopoletin is carried through the standard complexing procedure used to assay for histamine which includes measurements of fluorescing intensity at 440 nm. Therefore, it is likely that much of the apparent histamine reported to be present in cotton plant parts or cotton dusts is actually due to scopeletin.

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