Triclosan-resistant bacteria isolated from feedlot and residential soils

Abstract

Triclosan is an antimicrobial agent that is currently incorporated into hundreds of consumer and medical products. It can be either a bacteriostatic or bactericidal agent, depending on its formulation. It has activity against Gram-positive and Gram-negative bacteria, as well as some viruses and protists. The purpose of this study was to determine whether triclosan-resistant bacteria could be isolated from the soil. Soils from cattle feedlots and residential lawns were collected and assayed for the presence of these organisms by plating samples on growth media containing triclosan. Organisms were subsequently identified by partial 16S rRNA sequencing analysis. All the organisms isolated in this study were Gram-negative rods, with members of genus Pseudomonas being particularly well represented. This result may not be surprising because Gram-negative organisms are generally more resistant to triclosan, and since Pseudomonas bacteria are known to have numerous efflux mechanisms for dealing with harmful substances.

Keywords:

• Triclosan-resistance
• feedlots
• soils

Acknowledgments

The authors thank Sam Zwenger for collecting feedlot soil samples and Shingo Ishihara for expert laboratory assistance. This project was supported by the Kansas Idea Network for Biomedical Research Excellence Grant NIH P20RR016475 from the K-INBRE program of National Center for Research Success.