Fractionation and availability of heavy metals in tannery sludge-amended soil and toxicity assessment on the fully–grown Phaseolus vulgaris cultivars

Abstract

This study was conducted to assess the effect of tannery sludge on the bush bean (Phaseolus vulgaris) cultivars fully–grown on a culture sandy soil, as tannery sludge is valuable to improve soil fertility but long term studies evaluating the effect on fully grown plants are scarce. Tannery sludge amendments (0, 0.77, 1.54, 3.08 and 6.16 g tannery sludge kg⁻¹ soil) were characterized and the main heavy metals identified (Cr, Mn, Fe, K, and Zn) later on sequentially and singly extracted, for soil fractionation and availability determination, respectively. Metals showed different fractionation and availability patterns, being the most toxic metal (Cr) found to primarily bind to the carbonate fraction in soil, while almost 10% of the total Cr was available for plant uptake. In the green house experiments, bush bean cultivars exposed to increasing tannery sludge amendments were evaluated at different plant stages. Metal accumulation and physiological parameters (chlorophyll, carotenoids, nitrate reductase activity and dry weight) were determined. Toxicity was primarily due to Cr, stimulating or affecting the response of physiological parameters and suppressing seed formation at the highest tannery sludge ratio. Metals were mainly accumulated in the roots of bush beans, diminishing in the upper part of the plants with minimal translocation to seeds, supposing little risk for human consumption. Additionally, important correlations, antagonistic and synergistic relationships were observed between the extracted metals and metal accumulation in plant tissues.

Keywords:

• Tannery sludge
• chromium
• fractionation
• availability
• Phaseolus vulgaris
• accumulation
• toxicity

Acknowledgments

The authors gratefully acknowledge Dr. Rogelio Carrillo, Dr. Martha Barajas, Dr. Salvador Sánchez, Dr. Fernando Solís, Ms. Ricardo Acevedo, Ms. Alejandro Arcos and Bs. Ma Dolores Díaz for technical support. Finally JLL thanks the Consejo Nacional de Ciencia y Tecnología (Conacyt–México) for the Ph.D. scholarship. This work was financially supported by SEMARNAT–Conacyt–2002-C01-0184. In memoriam of ILC.