Biodegradation of methane, benzene, and toluene by a consortium MBT14 enriched from a landfill cover soil

Abstract

In this study, landfill cover soil was used as an inoculum source to enrich a methane, benzene, and toluene-degrading consortium MBT14. Under a single substrate, the maximum degradation rates of methane, benzene and toluene were 1.96, 0.15, and 0.77 mmole g-DCW⁻¹ h⁻¹, respectively. Although the coexistence of benzene and toluene inhibited the methane degradation rates, the consortium was able to simultaneously degrade methane, benzene and toluene. Methane had an insignificant effect on benzene or toluene degradation. Based on 16S rDNA sequencing analysis, Cupriavidus spp. are dominant in the consortium MBT14. The combined results of this study indicate that the consortium MBT 14 is a promising bioresource for removing CH₄, benzene, and toluene from a variety of environments.

Keywords:

• Methane
• methanotrophs
• VOC
• benzene
• toluene
• landfill.

Acknowledgment

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (MEST) (NRL program, R0A-2008-000-20044-0).