Predicting lead relative bioavailability in peri-urban contaminated soils using in vitro bioaccessibility assays

Abstract

In this study, lead (Pb) bioaccessibility was assessed in peri-urban contaminated soils using a variety of established in vitro assays. Bioaccessibility data was then used to predict Pb relative bioavailability (RBA) using published in vivo-in vitro regression models in order to compare calculated estimates and measured values. Lead bioaccessibility varied depending on the in vitro methodology employed with the relative bioavailability leaching procedure (RBALP) and in vitro gastrointestinal (IVG) assays providing more conservative Pb bioaccessibility values compared to those determined using PBET, UBM and Rel-SBRC-I assays. When Pb RBA was calculated, predicted values using PBET-G and UBM-G data were similar to measured Pb RBA values. However, Pb RBA was over-estimated by 1.6–5.5- and 2.6–6.6-fold when data and regression models from RBALP and IVG-G assays were employed.

Keywords:

• Bioaccessibility
• in vitro
• in vivo
• lead
• relative bioavailability

Acknowledgments

This research was funded by the Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC CARE) (Grant number 1-3-01-05/06). The authors would like to acknowledge the support of the Centre for Environmental Risk Assessment and Remediation (University of South Australia) and the Institute of Medical and Veterinary Science for this research.