Effects of titanium dioxide and zinc oxide nanoparticles on methane production from anaerobic co-digestion of primary and excess sludge

Abstract

Anaerobic co-digestion of primary and excess sludge is regarded as an efficient way to reuse sludge organic matter to produce methane. In this study, short-term and long-term exposure experiments were conducted to investigate the possible effects of titanium dioxide (TiO₂) and zinc oxide (ZnO) nanoparticles (NPs) on methane production from anaerobic co-digestion of primary and excess sludge. The data showed that TiO₂ NPs had no measurable impact on methane production, even at a high concentration (150 mg/g total suspended solids (TSS)). However, short-term (8 days) exposure to 30 or 150 mg/g-TSS of ZnO NPs significantly decreased methane production. More importantly, these negative effects of ZnO NPs on anaerobic sludge co-digestion were not alleviated by increasing the adaptation time to 105 days. Further studies indicated that the presence of ZnO NPs substantially decreased the abundance of methanogenic archaea, which reduced methane production. Meanwhile, the activities of some key enzymes involved in methane production, such as protease, acetate kinase, and coenzyme F₄₂₀, were remarkably inhibited by the presence of ZnO NPs, which was also an important reason for the decreased methane production. These results provide a better understanding of the potential risks of TiO₂ and ZnO NPs to methane production from anaerobic sludge co-digestion.

Keywords:

• Engineered nanoparticles
• enzyme activity
• inhibitory effect
• methanogenic archaea
• primary and excess sludge

Funding

This work was financially supported by the National Hi-Tech Research and Development Program of China (863 Program) (2011AA060903), the National Natural Science Foundation of China (51425802, 41301558, and 51278354), the Program of Shanghai Subject Chief Scientist (15XD1503400), and the Fundamental Research Funds for the Central Universities (2014KJ006).