Metal removal and morphological changes of B. megaterium in the presence of a spent catalyst

Abstract

Spent catalysts represent an environmental concern, mainly due to their elevated metal content. Although conventional treatment methods for spent catalysts are available, they generate large volumes of potentially harmful wastes and gaseous emissions. To overcome the environmental impact, biotechnological approaches are currently being explored and developed. Thus, the current study assayed the capability of Bacillus megaterium strain MNSH1-9K-1 to remove Al, Ni, V and Ti contained in the spent catalyst coded as ECAT-TL-II. To this end, B. megaterium MNSH1-9K-1 growth and metal uptake abilities in the presence of ECAT-TL-II spent catalyst at 15% (wt/vol) pulp density were evaluated in modified Starkey medium at 37 °C and 200 rpm. The results presented here show B. megaterium resistance capability to the high-metal content residue, and its Al, V and Ni removal ability, in 1,059.15 ± 197.28 mg kg^–1 of Al, 43.39 ± 24.13 mg kg^–1 of V and 0.58 ± 0.00 mg kg^–1 of Ni, corresponding to the 0.79%, 1.63% and 0.46% of each metal content, respectively, while no Ti removal was detected. Besides, it was observed that the sporulation process took place in B. megaterium cells in the presence of the spent catalyst. The results shown in this study suggest the potential of the strain MNSH1-9K-1 for the removal of metals contained in high-metal content residues, contributing also to the knowledge of the metal resistance and removal abilities of B. megaterium in the presence of a spent catalyst, and how morphological cell changes may be occurring while metal removal is taking place.

Keywords:

• Bacillus megaterium
• spent catalyst
• high-metal content residue
• metal removal
• metal resistance
• metal biosorption
• sporulation

Additional information

Funding

This project was supported by Grant No. 131203 from Consejo Nacional de Ciencia y Tecnología, Mexico.