Effect of short-term pig slurry amendment of soil on humified organic matter and its relationship with the dynamics of heavy metals and metals uptake by plants

Abstract

The impact of pig slurry (PS) application on the structural dynamics of humic substances (HS) and on the mobility of Cu, Zn, Ni, and Pb in a dystrophic Red Nitosol planted with winter forage grasses was evaluated. After four PS applications, the humic acids (HA) and fulvic acids (FA) were characterized by spectroscopy techniques allied to chemometrics methods. The metals contents in soil, in HS and in the tissues of plant were quantified. PS application increases the total organic carbon, especially the nonhumic carbon, which contribute to increase FA content. The carbon in FA and HA increases with the highest PS dose applied, especially aliphatic structures in FA and aromatic structures in HA. The amount of Pb and Cu in FA and HA increases respectively, as well as Cu, Zn, Ni, and Pb bioavailable. PS applications increase the biomass production in grasses and the metals content accumulated in the tissues. Our study shows that the PS application modifies the structure of SOM, incorporating fragments, and modifying its dynamics, which regulates the dynamics and the accumulation of metals in soils and plants. The association of metals with soluble structures seems to inactivate their toxicity and does not affect plant growth.

Keywords:

• Humic substances
• organic amendment
• spectroscopy
• chemometrics
• humic acid

Additional information

Funding

This work was supported by the Fundação Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, Brazil) under Grant no. 20128010, 1998025395; Conselho Nacional de Desenvolvimento Científico and Tecnológico (CNPq, Brazil) under Grant no. 306867/ 2018-4-PQ2; 303656/2015-8; and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil) under Grant no. 88882.332158/2019-01.