Recovery of poliovirus from sludge‐amended soils

Abstract

A method to recover enteroviruses from sludge amended soils was developed, using Poliovirus type 1 (strain LSc) as a model. Recovery efficiencies were evaluated for ratios of 1: 1, 1: 2.5, 1: 5, and 1: 10 of sludge amended soil to eluent, using 3% buffered beef extract at pH 9.5. Viral recoveries varied from 1.7 % to 98.8%, with a significant increase when larger volumes of the eluent were used. This study also showed that there is a linear correlation (r = 0.92) between the volume of the eluent and the percent recovery of poliovirus.

Key words:

• poliovirus
• enterovirus
• sludge
• soil
• land disposal