The effects of plant growth regulators on activity of eight serum enzymes in vitro

Abstract

In this study, the effects of plant growth regulators (PGRs), índole Acetic Acid (IAA), 2,3,5‐Triiodo Benzoik Acid (TIBA) and Kinetin on human serum enzymes, myocardial creatine kinase (CK‐MB), amylase, creatine kinase (CK), aspartate amino transferase (AST), serum glutamyl pruvic transferase (SGPT), alkaline phosphatase (ALK‐P), δ glutamyl transferase (GGT‐P) and lactate dehydrogenase (LDH) were investigated (in vitro). I₅₀ values of chemicals caused inhibition were determined by means of activity percentage ‐ [I] diagrams.

The results showed that IAA was an activator rather than an inhibitor. It only inhibited AST activity, but increased activities of CK, CK‐MB, amylase, ALK‐P and LDH. However, IAA ineffected on SGPT and GGT‐P. Both TIBA and Kinetin inhibited CK‐MB, but either chemicals ineffected on CK, amylase, ALK‐P, GGT‐P. On the other hand, TIBA ineffected on SGPT and LDH, while Kinetin increased activity of the same enzymes. In conclusion, it was showed that the studied chemicals weakly inhibited enzymes.

Key words:

• Serum enzymes
• plant growth regulators
• human