A rapid fibroblast culture technique for high resolution karyotypes

Abstract

For chromosomes studies in mammals, we developed a rapid fibroblast’ culture techique which is particularly advantageous for small mammals when sacrifice is not desirable. Small exterior biopsies are sequentially digested in EDTA/Trypsin and collagenase. Cell cultures can be subdivided and harvested in about one week, compared to the 3–4 week period normally required. The use of antibiotics limits the inconvenience of microbial contamination and permits, transport of samples from the field to the laboratory over a period of one week.

Key words:

• Mammals
• Chromosomes
• Cell culture techniques