Morphogenic response in vitro of epicotyl segments of Citrus macrophylla

Abstract

The influence of explant orientation, contact of the cut surface with the medium, type of growth regulators and distance of epicotyl cuttings from the cotyledonary node (the part of the seedling where the cotyledons are attached) on in vitro morphogenesis of Citrus macrophylla (alemow) was evaluated. Maximum number of shoots was induced from epicotyl segments placed horizontally.

Keywords:

• Adventitious shoots
• Citrus macrophylla
• cytokinins
• explant orientation
• morphogenesis
• organogenesis

Acknowledgements

Authors thank Dr. S. Recupero and Dr. R. Zurru for supplying the seeds used in this research.