Mineral element distribution in organs of dual-toxin transgenic (Bt+CpTI) cotton seedling

Abstract

Being a new cultivar, the physiology of transgenic cotton, especially dual-toxin transgenic (Bt+CpTI) cotton, is not yet completely understood. Twelve elements in three organs of dual-toxin transgenic cotton seedlings were analyzed by ICP-MS. The distributions of the 12 elements were substantially different from those of non-transgenic cotton. In particular, the contents of B, Mg, P, K and Ca were the highest in leaves, while those of Si, Fe, Rb and Cu were the highest in roots; other elements had similar contents in the two organs, which were higher than those in the stem. Compared with non-transgenic cotton, the 12 elements could be classified into four groups according to their contents and distributions in the three organs: (a) P, K and Cu: their contents in transgenic cotton were remarkably lower, especially contents of P and K in leaves that were one times lower than those in leaves of non-transgenic cotton; (b) B, Mg and Mo: their contents in leaves and roots of transgenic cotton were higher, but lower in stems, compared with non-transgenic cotton; (c) Si, Mn, Fe, Rb and Zn: compared with non-transgenic cotton, these were lower in leaves and stems, but higher in roots of transgenic cotton; and (d) Ca: compared with non-transgenic cotton, its content was higher in all three organs of the transgenic counterpart. The decrease in soluble proteins and the expression of Bt and CpTI genes could be responsible for these changes. Further studies are needed to verify this hypothesis.

Keywords:

• Organ distribution
• mineral elements
• transgenic cotton
• BT toxin

Acknowledgements

This research was supported by the Program for Changjiang Scholars and Innovative Research Team in the University (No. IRT0511), and the Natural Science Foundation of China (No. 70573111).