Cross-amplification of nonnative Acacia species in the Hawaiian Islands using microsatellite markers from Acacia koa

Abstract

Thirty-one microsatellite markers, developed from Acacia koa, were evaluated for cross-amplification of seven nonnative species of Acacia in the Hawaiian Islands. The number of alleles per locus ranged from one to seven, with 16 of the 31 markers showing polymorphism in at least two of the nonnative Acacia species. Of the Acacia species examined, A. melanoxylon showed the highest percentage of amplified loci.

Keywords::

• Acacia koa
• cross-amplification
• microsatellite markers
• tree legume

Acknowledgements

We thank Dr J.B. Friday, Dr Russell Yost, Dr James Leary, Steve Smith, Iris Teruya, and Aileen Yeh for their technical support. This research was supported by United States Department of Agriculture-Tropical and Subtropical Agricultural Research (USDA-TSTAR) Award No. 2009-04862 and partially by the McIntire-Stennis Project No. HAW00597-M.