Evidence of a new Hg-tolerant ecotype of Rumex induratus from Almadén (Ciudad Real, Spain)

Abstract

Mercury tolerance in wild vascular plants has hardly been studied and a tolerant ecotype is not known. In order to confirm the tolerance to Hg of Rumex induratus naturally growing in the biggest Hg mine in the world (Almadén population), the population was compared in a hydroponic experiment with another population from a non-Hg-contaminated area (Colmenar). The plants were exposed to different doses of Hg and a dose of As to establish whether the tolerance to Hg coincides with tolerance to other trace elements. Plants from Colmenar reached up to 1322 μg Hg g^− 1 in roots and 65 μg Hg g^− 1 in shoots and showed a significant decrease in biomass due to Hg exposure, whilst Almadén accumulated only 812 μg Hg g^− 1 and 56 μg Hg g^− 1 in roots and shoots, respectively. The Almadén population showed a higher tolerance to intense exposure to Hg, but not to As. Plants from Almadén exposed to Hg showed higher capacity to synthesise thiols in the root and to control oxidative stress and Zn starvation. Our findings suggest that R. induratus could be used to enhance understanding of the mechanisms of Hg tolerance in plants.

Keywords::

• Mercury phytostabilisation
• tolerance
• oxidative stress
• As uptake
• Zn uptake

Acknowledgements

This study was supported by Spanish Ministry of Education and Science, Project CGL2009-13171-C03-02 and by Comunidad de Madrid, Project EIADES (S2009AMB-1474). Authors thank Roberto Bayón for his assistance in the hydroponic experiment and Maren Flagmeier and Aoife Brennan for their assistance with the English language.

Notes

^1. Email: [email protected]; [email protected]

^2. Email: [email protected]