Identification of culturable bacterial endophyte community isolated from tissues of Vitisvinifera “Glera”

Abstract

Endophytes are micro-organisms that colonize the internal tissues of plants without inducing signs of negative effects and that can provide benefits to plant health and yield. In the present work, the culturable bacterial endophyte community, colonizing vegetative organs of grapevine, was isolated from surface-sterilized plant tissues and characterized by molecular methods. From roots, shoots and leaves of Vitis vinifera “Glera”, located in six different vineyards throughout the Conegliano-Valdobbiadene DOCG area (Veneto, Italy), 381 culturable strains were successfully isolated; amplified ribosomal DNA restriction analysis and nucleotide sequencing showed that approximately 30% of the endophyte community belonged to the genus Bacillus, which was the most represented; other genera such as Staphylococcus, Microbacterium, Paenibacillus, Curtobacterium, Stenotrophomonas, Variovorax, Micrococcus and Agrococcus were identified. Endophyte community composition within each vine was different in respect to other endophyte populations living in grapevine plants coming from different vineyards; moreover, the bacterial composition changed depending on the season of sampling. The above data highlight the great diversity of culturable bacterial species inhabiting Glera grapevines and open the way for a characterization and selection of strains that could potentially be used to improve the vineyard management for plant growth and yield, plant responses to stresses, biocontrol and biofertilization.

Keywords::

• ARDRA
• culturable bacteria
• endophyte
• grapevine
• plant–microbe interaction

Acknowledgements

This work is part of an F.S.E. research project in collaboration with the Consorzio per la Tutela del Conegliano Valdobbiadene DOCG; E.B. is the recipient of Assegno di Ricerca Junior of Padova University: [Project 2011-prot. CPDR114442]. We thank COST ACTION FA1103 for supporting this work.