Photoperiod buffers responses to salt and temperature during germination of two coastal salt marsh colonizers Juncus acutus and Juncus maritimus

Abstract

Juncus acutus and J. maritimus are two colonizers of coastal marsh rangeland with worldwide distribution. We tested the effect of salt, temperature, and photoperiod on the germination capacity of seeds of the two Juncus from the Rhône delta (south of France). We measured the first day of germination, the mean time, speed, and rate of germination on seeds subjected to five salinity levels, three Dark–Light temperatures and two D-L photoperiods (12–12 and 10–14). The 10–14 D-L photoperiod, although it corresponds to one of the two main germination periods of J. acutus and J. maritimus in the northern Mediterranean, has never been previously used to study their germination capacity. Analyses showed significant effects of salinity and temperature on the germination parameters tested, and for these two factors we found results comparable to those of previous studies. Salinity slowed down and reduced the germination process while spring temperatures had a positive effect. A surprising result was obtained by changing the D-L photoperiod from 12–12 to 10–14 which then largely buffered the negative effect of salt and high temperatures on germination. This capacity of J. acutus and J. maritimus to withstand salty conditions during the germination phase due to the spring photoperiod could be decisive in the ability of both species to colonize saline environments.

Keywords::

• Halophyte
• photoperiod
• recruitment
• salinity
• temperature

Acknowledgements

This work was supported by the Région Provence-Alpes-Côte d'Azur, the Foundation ProValat and the Foundation MAVA. We are grateful to Olivier Boutron, Diedre Flanagan, Patrick Grillas, Gaétan Lefèbvre, Léa Mishler, Marta Siliato and Loïc Willm for their help at different stages of the work.