Indirect organogenesis from leaf explants of Hemidesmus indicus (L.) R. Br.: an important medicinal plant

Abstract

Hemidesmus indicus (Asclepiadaceae) leaf explants were utilized for establishing culture in MS medium fortified with individual cytokinins, auxins, and their combinations. Optimum response (80%) was observed in N⁶-benzyladenine (BA, 20 μM) + indole-3-acetic acid (IAA, 1 μM) with 19.67 ± 0.81 shoots per explant. Roots were induced in ¼MS + indole-3-butyric acid (IBA, 20 μM).

Keywords:

• Hemidesmus indicus
• indirect organogenesis
• leaf explant
• Murashige and Skoog’s medium
• shoot cultures

Funding

This work was supported by the University Grants Commission, New Delhi, India, under Research Fellowship in Science for Meritorious Students (UGC-BSR fellowship) scheme to A.R. Pathak [grant number F.No.7-319/2011(BSR)].