Genetic diversity analysis of 60 ginger germplasm core accessions using ISSR and SSR markers

Abstract

Molecular techniques play a critical role in studies of phylogeny and, thus, have been applied to understand the distribution and extent of genetic variation within and between species. In the present study, a genetic analysis was undertaken using molecular markers (9 ISSR and 13 SSR) on 60 ginger cultivars from different regions of the eastern coast of India (Odisha). The data obtained with 22 polymorphic markers revealed moderate to high diversity in the collection. Both ISSR and SSR markers were efficient in distinguishing all the 60 ginger cultivars. A total of 42 and 160 polymorphic bands were observed with ISSR and SSR markers, respectively. However, SSR markers were observed to be better at displaying average polymorphism (63.29%) than ISSR markers (55%). Analysis of molecular variance results showed that 52 and 66% of the variation occurred among different ginger populations, whereas 48 and 34% of the variation was found within populations, respectively, using ISSR and SSR markers, indicating that ginger cultivars display significant genetic diversity at the population level. Principal coordinates analysis and the dendrogram constructed out of combined data of both markers showed grouping of ginger accessions to their respective area of collection, indicating geographical closeness due to genetic similarity irrespective of the relationship that exists at the morphological level.

Keywords:

• Ginger
• genetic diversity
• molecular markers
• PCoA
• AMOVA

Acknowledgements

We owe thanks to our President, Prof. M.R. Nayak and Dean, Prof. S.C. Si, Centre of Biotechnology, SOA University, Bhubaneswar for providing infrastructure and encouragement. We acknowledge the timely supply of released ginger cultivars by Mr P. Sial, in-charge HARS, OUAT, Pottangi.