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ABSTRACT
Coxiella burnetii is the cause for Q- fever that is spread all over the world and affects both, animals and humans. Q fever is a disease of great significance which may take a chronic or even lethal course. Coxiella burnetii is a potential biological agent that is possible to be used as a biological weapon. Prompt and specific diagnostic tools as well as elaborating new detection methods are needed for an exact diagnosis and a correct etiological treatment. One of the most contemporary molecular—biological methods for a prompt and specific detection at genetic level is the real-time Polymerase Chain Reaction (PCR). LUX primers are used instead of conventional ones and a new technology for olygonucleotides marking is applied. We have constructed LUX Primer System with the support of D-LUX TM Designer and have successfully tested its specificity, sensitivity and effectiveness in sequence detecting of Coxiella burnetii genome which is strongly peculiar to that kind of microorganism.