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ABSTRACT
A successful immobilization in chitosan of the lactic acid bacteria Lactobacillus acidophilus A., Lactobacillus helveticus H., Lactobacillus casei subsp. casei C. and Lactobacillus plantarum 226–15 was accomplished and high viable cell counts (∼1010 cfu/g) in the gel beads were obtained. The resistance of the included in the gel matrix cells to model conditions of digestion was investigated. The high resistance of the immobilized cells to low and neutral pH values and the presence of pepsin and high concentrations of bile salts were demonstrated. The immobilized in chitosan cells of all of the studied strains showed a significantly higher resistance when compared to free growing cells. Thus, they can successfully be used for the production of probiotic preparations.