Design and Evaluation of New Light-Upon-Extension (Lux) Real-Time PCR Primer System for Detection of Polyomavirus Hominis 2 (JC Virus) in Clinical Samples

ABSTRACT

We describe the design, optimization and application of highly effective primer systems for detection of Polyomavirus hominis 2 (JCV) using Light Upon Extension (LUX) Real-time PCR. Our results confirm the high specificity and also the rapidity and non-invasiveness of the methodfor demonstration of ongoing viral replication. We believe that the LUX technology is comparable to the probe based real-time PCR assays for detection of human viruses, such as TaqMan, but has an advantage in terms of cost-effectiveness, convenience and the possibility for a subsequent dissociation analysis.

Keywords:

• Light-Upon Extension (LUX) real-time PCR
• Polyomavirus hominis 2
• JCV