Similarities in mRNA expression of peripheral blood drug metabolizing enzymes and cancer marker genes with biopsy samples of head and neck cancer patients

Abstract

Purpose: To develop peripheral blood mRNA expression profiles of drug metabolizing enzymes (DMEs) as a surrogate to monitor tobacco induced head and neck squamous cell carcinoma (HNSCC), attempts were made to investigate (i) similarities in alterations with the cancer marker genes in biopsy samples and (ii) if alterations similar to that seen in biopsy samples are reflected in peripheral blood.

Methods: Total RNA from eight soft gingival tissues and eight biopsy samples of HNSCC patients and total DNA and RNA from blood of healthy controls (n = 150) and HNSCC patients (n = 150) was processed for expression and genotyping studies. Blood from patients receiving chemo-radiotherapy was processed for follow-up study.

Results: qRT-PCR revealed significant increase in mRNA expression of DMEs in biopsy and blood samples of HNSCC patients when compared to controls. Similar alterations were observed in cancer marker genes in these samples. Patients with variant genotypes of DMEs showed greater magnitude of alterations in mRNA expression when compared to wild type controls. Responders of chemo-radiotherapy showed significant decline in induction of mRNA expression of DMEs and cancer marker genes

Conclusions: The data suggest that peripheral blood expression profiles could be used to monitor tobacco-induced HNSCC as well as the treatment response.

Keywords:

• HNSCC
• tobacco
• blood
• mRNA
• biomarker
• CYPs
• genotype

Acknowledgements

The authors are grateful to the Director, Indian Institute of Toxicology Research (Council of Scientific & Industrial Research, CSIR), Lucknow for his keen interest and support in carrying out the study.

Disclosure statement

The authors report no conflicts of interest.

Additional information

Funding

The financial assistance of Indo-US Project on Identification and validation of early biomarkers for predicting toxicity including pre-carcinogenic lesions in individuals exposed occupationally to polycyclic aromatic hydrocarbons (PAHs) and through tobacco use (No. 68/1/2012-NCD-1-Indo-US project) sponsored by ICMR, New Delhi, and INDEPTH (BSC0111), CSIR for carrying out the above study is gratefully acknowledged. CSIR-IITR Communication No.: 3423.