NCR3LG1 (B7-H6) is a potential prognostic factor for bladder cancer patients

Abstract

Background

NCR3LG1 (B7-H6) protein is selectively overexpressed on tumour and is associated with fatal disease progression of various cancer. However, its prognostic value in bladder cancer (BCa) has not been well elaborated.

Methods

We examined the expression of NCR3LG1 in human BCa and analysed its clinical significance and prognostic value. Meanwhile, the expression of NCR3LG1 was intervened in human BCa cell line 253JBV to analyse subsequent effects on tumour.

Results

According to TCGA data, the disease-free survival rate was statistically significant between the NCR3LG1 high expression group and the low expression group (Log Rank p = 0.006). Immunohistochemical staining showed that the expression of NCR3LG1 in BCa tissue was significantly higher than that in adjacent tissues (p < 0.0001), which was positively correlated with TNM staging (p = 0.008), histological grade (p = 0.022), and lymphoma metastasis of BCa (p = 0.032). The proliferation (p < 0.0001), invasion (p < 0.001) and migration ability (p < 0.001) of 253JBV cells are significantly inhibited by knocking down the expression of NCR3LG1, and the cell cycle arrest is induced at the G1 phase, which accelerates the apoptosis of BCa cells (p < 0.005).

Conclusion

Our findings indicate that NCR3LG1 is involved in the progression of human BCa and may become a potential prognostic biomarker for BCa.

Keywords:

• bladder cancer
• cancer progression
• NCR3LG1
• biomarker

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The present study was funded by Key laboratory research fund of the second hospital of Tianjin medical university [grant no.2017ZDSYS12], the Natural Science Foundation of Tianjin [grant no. 16JCYBJC26500] and the National Natural Science Foundation of China [grant no. 81301949].