Downregulation of fibrosis and inflammatory signalling pathways in rats liver viaPulicaria crispa aerial parts ethanol extract

Abstract

Context

Liver is a vital organ for the detoxification of toxic substances in the body, where fibrosis is the major cause of liver damage. Pulicaria crispa processes many therapeutic applications such as antioxidant, antimicrobial, anticancer and anti-ulcerative agent.

Objective

This study aimed to modulate the fibrosis and inflammatory signalling pathways in carbon tetrachloride (CCl₄)-induced liver damage in rats via treatment with Pulicaria crispa aerial parts ethanol extract (PCEE).

Materials and methods

CCl₄ was intraperitoneally injected at a dose of 0.5 mL/kg b.wt./twice a week/six consecutive weeks, PCEE was orally allocated at a dose of 250 mg/kg b.wt./day/six weeks and silymarin was orally administrated at a dose of 100 mg/kg, b.wt/day/six weeks. The plant extract evaluation was done through measuring aspartate and alanine aminotransferases (AST& ALT), alkaline phosphatase (ALP), total lipids (TP), total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low level glycoprotein-cholesterol (LDL-C), alpha fetoprotein (AFP), reduced glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), tumour necrosis factor-α (TNF-α) and interleukin 6 (IL-6). The liver architectures were also estimated.

Result

The phytochemical analysis of the extract showed the presence of sterols and/or triterpenoids. Treatments with plant extract suppressed significantly (p < 0.0001) the levels of AST, ALT, ALP, TP, TG, TC, LDH-C, MDA, NO, AFP, TNF-α and IL-6, while increased (p < 0.0001) the levels of HDL-C, GSH and SOD. The histopathological features confirmed the therapeutic role of the plant extract.

Conclusion

PCEE succeeded to exert anti-fibrotic, anti-inflammatory and anti- oxidants effects in CCl₄-induced liver fibrosis.

Keywords:

• Pulicaria crispa
• CCl₄
• fibrosis
• antioxidant
• inflammatory cytokines

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

Data in this manuscript is available to any reader upon acceptance.