Chemical compositions of Commiphora opobalsamum stem bark to alleviate liver complications in streptozotocin-induced diabetes in rats: Role of oxidative stress and DNA damage

Abstract

Context

Hyperglycaemia plays an important role in the development of non-alcoholic fatty liver disease, which is a common complication in diabetics.

Objective

The present study aimed to investigate the chemical composition and the efficacy of Commiphora opobalsamum stem bark butanol fraction in ameliorating liver injury associated with diabetes induced by streptozotocin (STZ) in rats.

Materials and methods

The butanol fraction was applied to high-performance liquid chromatography-mass spectrometry (HPLC/MSⁿ) to identify the most bioactive metabolites. Diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg body weight), while treatment with the plant extract was performed (100 mg/kg body weight) for three weeks after diabetic induction for one month.

Results

Thirty eight metabolites were tentatively identified from the butanol fraction of C. opobalsamum stem bark. Insulin, glutathione, superoxide dismutase, and high density lipoprotein levels in diabetic rats were significantly low (p < 0.05), while glucose, α-amylase, malondialdehyde, aspartate and alanine aminotransferases, cholesterol, triglycerides, low density lipoprotein, tumour necrosis factor-α, interleukin-6, and DNA fragmentation levels were significantly high. Treatment with the plant extract showed improvements in the seleced parameters by variable degrees. Conclusion: The plant extract is considered as a promising natural therapeutic agent against liver injury, hyperglycemia, oxidative stress, inflammation, hyperlipidaemia, and DNA damage.

Graphical Abstract

Keywords:

• Diabetes
• Commiphora Opobalsamum
• LC/MSⁿ
• liver injury
• diabetes
• oxidative stress
• inflammation

Acknowledgments

The authors would like to express their deepest thanks to National Research Centre, Egypt, for facilitating this work.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

Data in this manuscript is available to any reader upon acceptance.

Funding

The author(s) reported there is no funding associated with the work featured in this article.