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Original

Cerebrospinal fluid from human immunodeficiency virus–infected individuals facilitates neurotoxicity by suppressing intracellular calcium recovery

, , &
Pages 144-156 | Received 17 Mar 2004, Accepted 01 Nov 2004, Published online: 10 Jul 2009
 

Abstract

Neurologic decline associated with penetration of human immunodeficiency virus type 1(HIV-1) into the central nervous system is thought to be due, in large part, to inflammation and local secretion of neurotoxic substances. To examine the cellular processes that mediate neurotoxicity in vivo, the authors valuated the ability of neurons to maintain intracellular calcium homeostasis in the presence of toxic cerebrospinal fluid (CSF) (CSFtox) collected from a subset of HIV-infected individuals. Exposure of rat neural cultures to CSFtox resulted in a gradual increase in intracellular calcium in neurons (+63%), microglia (+251%), and astrocytes (+52%). Pretreatment of neural cultures with CSFtox resulted in an exaggerated calcium response to a brief pulse of glutamate and a > 90% suppression of the rate of recovery of intracellular calcium. Attempts to model the deficit using inhibitors of calcium transport across endoplasmic reticulum, mitochondrial, or plasma membrane indicated that blockade of the plasma membrane sodium/calcium exchanger was best able to reproduce the deficits seen during exposure to CSFtox. Because the inability of cells to maintain calcium homeostasis would lead to exaggerated responses from a wide variety of stimuli, therapeutics designed to facilitate calcium transport from the cell may provide more comprehensive and effective intervention than strategies targeted to specific receptor pathways.

This work was supported by NIH grats MH62690, AI47749, and the UNC center for AIDS Research (P30-HD37260). Received 17 March 2004; revised 25 August 2004; accepted 1 November 2004.

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