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Medical Mycology Volume 47, 2009 - Issue 6
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Review Article

Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii

Wieland Meyer Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, University of Sydney Western Clinical School at Westmead Hospital, Westmead, Australia
,
David M. Aanensen Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, UK
,
Teun Boekhout CBS Fungal Biodiversity Centre, Utrecht, The Netherlands
,
Massimo Cogliati Laboratory of Medical Mycology, Dip. Sanitá Pubblica-Microbiologia-Virologia, Università degli Studi di Milano, Milano, Italy
,
Mara R. Diaz Rosentiel School of Marine and Atmospheric Science, University of Miami, Miami, Fl, USA
,
Maria Carmela Esposto Laboratory of Medical Mycology, Dip. Sanitá Pubblica-Microbiologia-Virologia, Università degli Studi di Milano, Milano, Italy
,
Matthew Fisher Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, UK
,
Felix Gilgado Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, University of Sydney Western Clinical School at Westmead Hospital, Westmead, Australia
,
Ferry Hagen CBS Fungal Biodiversity Centre, Utrecht, The Netherlands
,
Sirada Kaocharoen Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, University of Sydney Western Clinical School at Westmead Hospital, Westmead, Australia; Inter-Department of Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok, Thailand
,
Anastasia P. Litvintseva Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA
,
Thomas G. Mitchell Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA
,
Sitali P. Simwami Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College London, London, UK
,
Luciana Trilles Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, University of Sydney Western Clinical School at Westmead Hospital, Westmead, Australia; Laboratório de Micologia, Instituto de Pesquisa Clínica Evandro Chargas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil
,
Maria Anna Viviani Laboratory of Medical Mycology, Dip. Sanitá Pubblica-Microbiologia-Virologia, Università degli Studi di Milano, Milano, Italy
&
June Kwon-Chung Molecular Microbiology Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH, Bethesda, MD, USACorrespondence[email protected]
 show all
Pages 561-570 | Received 27 Jan 2009, Published online: 01 Sep 2009
 

Abstract

This communication describes the consensus multi-locus typing scheme established by the Cryptococcal Working Group I (Genotyping of Cryptococcus neoformans and C. gattii) of the International Society for Human and Animal Mycology (ISHAM) using seven unlinked genetic loci for global strain genotyping. These genetic loci include the housekeeping genes CAP59,GPD1, LAC1, PLB1, SOD1, URA5 and the IGS1 region. Allele and sequence type information are accessible at http://www.mlst.net/.

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