Reversal of high fat diet-induced obesity through modulating lipid metabolic enzymes and inflammatory markers expressions in rats

Abstract

In this study, we evaluated the ameliorative potential of Cucurbita maxima seeds oil (CSO (100 mg/kg body weight)) supplementation to high fat diet (HFD)-induced obese rats for 30 days on the changes in body weight, markers of lipid metabolism such as LDL, HDL, triglycerides, total cholesterol, adiponectin, leptin, amylase, and lipase. We also investigated the effects of CSO on the changes of lipid metabolic enzymes such as fatty-acid synthase, acetyl CoA carboxylase, carnitine palmitoyl transferase-1, HMG CoA reductase, and inflammatory markers (TNF-α and IL-6). Administration of CSO revealed significant diminution in body weight gain, altered the activity, expressions of lipid marker enzymes and inflammatory markers. It demonstrated that CSO had considerably altered these parameters when evaluated with HFD control rats. In conclusion, this study suggested that CSO might ameliorate the HFD-induced obesity by altering the enzymes and mRNA expressions important to lipid metabolism.

Keywords:

• Cucurbita seed oil
• obesity
• metabolic disorders
• lipid metabolism
• high fat diet

Acknowledgements

The authors thank K.S.R. College of Technology, Tiruchengode, Tamil Nadu, India, for providing facilities to do animal studies and also express heartfelt thanks to the management of K. S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Tamil Nadu, for their support and encouragement.

Disclosure statement

No potential conflict of interest was reported by the authors.

The authors would like to inform that this is a self-funded study and has no conflict of interest.