The impact of concomitant administration of vanadium and insulin on endothelial dysfunction markers (PAI-1 and ET-1) in type 1 diabetic rats

Abstract

Endothelial dysfunction in type 1 diabetes mellitus (T1DM) is an important factor in the pathogenesis of micro- and macrovascular complications. The present study was to investigate the impact of combined vanadium and insulin for proper control and protection against endothelial dysfunction in T1DM rats. Sixty male Sprague-Dawley rats were randomly divided into six groups; control non-treated; control vanadium treated; T1DM; T1DM + insulin; T1DM + vanadium; T1DM + insulin + vanadium treated groups. At the end of the experiment (6 weeks), serum C-reactive protein, tumour necrosis factor-alpha, IL-6, endothelin-1, plasminogen activator inhibitor-1, fasting glucose serum lipogram, liver homogenate SOD activity and MDA levels were determined. Concomitant insulin and vanadium treatment improved the diabetic metabolic disturbances in addition to endothelial dysfunction and inflammatory markers. We can conclude that concomitant administration of both vanadium and insulin in T1DM decreased the risk for the development of endothelial dysfunction, micro- and macrovascular complications.

Keywords:

• Endothelial dysfunction
• inflammatory markers
• vanadium
• plasminogen activator inhibitor-1
• endothelin

Acknowledgements

The authors highly appreciate the extreme help of all staff members of Abha General Hospital. The authors also thank greatly Dr. Khalid S Bashir, Ahmed M Darwesh, and Dr. Omar M Morsy for their help in the collection, processing of samples and performing the statistical analysis of data. We extend our thanks to all staff members and technicians of the Physiology and Biochemistry Departments of Colleges of Medicine, King Khalid University, Saudi Arabia, and College of Medicine, Menoufia University, Egypt; for their great help in this work.

Disclosure statement

No potential conflict of interest was reported by the authors.