Investigation of healing effects of lemon (Citrus limonum) seeds lyophilized extracts on experimental diabetic rats

Abstract

In this study, the effects of lemon seed lyophilised extract (LSLE) were investigated on the diabetic rats. Groups were conducted as normal control (NC), diabetic control (DC), diabetic + 20 mg acarbose/kg bw (DAC)), diabetic + 100 mg LSLE/kg bw (DLSLE1), diabetic + 200 LSLE mg/kg bw (DLSLE2) and diabetic + 400 LSLE mg/kg bw (DLSLE4). The protective and antioxsidant effects of LSLE on experimental diabetes complications were evaluated by measuring hepatic and renal damage biomarkers (HRDBs), antioxidant defence system constituents (ADSCs), diabetes biomarkers and MDA content in tissues of diabetic rats. Glucose, HRDBs, HbA1c, lipid profile (LP) levels increased in DC compared to NC whereas these parameters of the supplementation groups showed a significant decreas compared to DC. Also, it was determined an increase MDA content and fluctuate ADSCs in the DC tissues whereas the LSLE restored the parameters towards to the NC. It can be said that LSLE is may have healings effects against diabetic complications.

Keywords:

• Lemon seed
• antidiabetic
• antioxidant
• hepatoprotective

Compliance with ethical standards

Research involving human participants and/or animals

Wistar female rats 3–4 months of age with an average weighing 200–300 g were provided with the Experimental Animal Research Centre of Yuzuncu Yil University. Rats were in housed controlled humidity (60–70%) and temperature (25 ± 1 °C) conditions with a 12 h light/dark cycle. All animals were fed a based standard diet and water ad libitum in stainless cages. The rats received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” prepared by the National Academy of Science and published by the National Institute of Health. The ethic regulations were followed in accordance with national and institutional guidelines for the protection of animal welfare during experiments. The ethic committee of the Yuzuncu Yil University approved the study with 26 October 2016–2016/10 protocol number.

Informed consent

No human participants involved in the present research.

Disclosure statement

None of the authors has a conflict of commercial interest such financial and nonfinancial interest and other relationship with manufacturers of pharmaceuticals, laboratory supplies and medical devices. The authors are grateful to the University of Van Yuzuncu Yil Grant Commission for providing financial assistance of the research (Project number: VYY€UBAP-FYL-2017–5690).