Protective effect of saxagliptin against renal ischaemia reperfusion injury in rats

Abstract

Saxagliptin is an effective and selective dipeptidyl peptidase-4 (DPP-4) inhibitor. This study was designed to determine possible protective effects of saxagliptin against damage caused by renal ischaemia/reperfusion (I/R) in rats. In this study, 40 rats were divided into 4 groups (n = 10 for each). Group 1 (Control), Group 2 (I/R) in both kidneys ischaemia of 45 min was performed, and then reperfusion was applied for 24 h. Saxagliptin (Group 3: 2 mg/kg and Group 4: 10 mg/kg) was administered by oral gavage to the animals in treatment groups, before the I/R. Saxagliptin decreased the markers (BUN, Cre, NGAL, KIM-1 and IL-18) of acute renal damage in blood and kidney tissue. Saxagliptin provided increase in antioxidant enzyme levels and decrease in MDA and apoptosis. Histological results showed that the administration of saxagliptin exhibited a protective effect against renal damage caused by I/R. These results indicates that saxagliptin provide protection against kidney injury caused by I/R.

Keywords:

• Dipeptidyl peptidase-4 inhibitor
• oxidative stress
• saxagliptin
• renal ischaemia reperfusion
• lipid peroxidation

Compliance with ethical standards

The study was ethically approved and registered by Inonu University, Faculty of Medicine, Experimental Animal Ethics Committee (Protocol No: 2017/A-28).

Author contributions

ST, AB and SS conceived the study idea; MC, AT and YE contributed to the study design. All authors contributed towards data collection. ST, YE and CT analysed the data and wrote the manuscript. All authors discussed the data and approved the submitted draft. SS takes responsibility for the integrity of the study.

Disclosure statement

The authors declare no conflicts interest.

Additional information

Funding

This study was supported by Inonu University Scientific Research Projects Fund [Project # TCD-2017–647].