Exercise training decreases oxidative stress in skeletal muscle of rats with pulmonary arterial hypertension

Abstract

The effects of exercise training on oxidative stress in gastrocnemius of rats with pulmonary hypertension were studied. Four groups were established: sedentary control (SC), sedentary monocrotaline (SM), trained control (TC), trained monocrotaline (TM). Exercise was applied for 4 weeks, 5 days/week, 50–60 min/session, at 60% of VO₂ max. Right ventricular (RV) pressures were measured, heart and gastrocnemius were removed for morphometric/biochemical analysis. Lipid peroxidation (LPO), H₂O₂, GSH/GSSG, and activity/expression of antioxidant enzymes were evaluated. Increased RV hypertrophy, systolic and end-diastolic pressures (RVEDP) were observed in SM animals, and the RVEDP was decreased in TM vs. SM. H₂O₂, SOD-1, and LPO were higher in the SM group than in SC. In TM, H₂O₂ was further increased when compared to SM, with a rise in antioxidant defences and a decrease in LPO. GSH/GSSG was higher only in the TC group. Exercise induced an efficient antioxidant adaptation, preventing oxidative damage to lipids.

Keywords:

• Monocrotaline
• aerobic training
• pulmonary hypertension
• gastrocnemius
• reactive oxygen species

Acknowledgements

Financial support from the National Council of Technological and Scientific Development (CNPq), Foundation for Research of the State of Rio Grande do Sul (FAPERGS), and Coordination for the Improvement of Higher Education Personnel (CAPES).

Disclosure statement

No potential conflict of interest was reported by the author(s).