Aberrant methylation of miR-124 upregulates DNMT3B in colorectal cancer to accelerate invasion and migration

Abstract

The dysregulation of microRNA expression is significantly associated with the initiation and development of CRC. miR-124 is markedly downregulated in colorectal cancer. In the present study, the effects of methylation, over expression and downregulation of miR-124 and its target gene DNMT3B on the proliferation, migration and invasion of colorectal cell line were investigated. The promoter methylation status of miR-124 in the CRC was investigated by methylation specific PCR (MSP). The potential role of miR-124 expression in CRC cells was investigated using the demethylation reagent 5-Aza-CdR and transfection of miR-124 mimic/antimir. MSP revealed that miR-124 promoter region was hypermethylated, result in its significant downregulation in tumour tissues. We showed miR-124 expression was upregulated following 5-AZA-CdR treatment. Transfected Hct-116 cell line with miR-124 leads to decreased DNMT3B expression, cell proliferation, migration and invasion of HCT-116. In conclusion, our data indicate that miR-124 suppress colorectal cancer proliferation, migration and invasion through downregulating DNMT3B level.

Keywords:

• Colorectal cancer
• miR-124
• DNMT3B
• hypermethylation
• invasion

Disclosure statement

The authors declare there is no conflict of interest.

Additional information

Funding

This work was financially supported by grant [96-01-30-34250] from the Deputy of Research, Tehran University of Medical Sciences.