Constant-moderate versus high-intensity interval training on heart adiponectin levels in high-fat fed mice: a preventive and treatment approach

Abstract

Context

Research has described that adiponectin plays a key role in cardiomyocytes metabolism, however, the effects of exercise during obesity on cardiac adiponectin levels is unclear.

Objective

To investigate the effects of constant-moderate endurance (END) and high-intensity interval training (HIIT), on heart adiponectin levels in mice.

Material and methods

Two experiments were conducted: (1) preventive (EX1): 10 week-old male mice were fed standard (CHOW) or high-fat diet (HFD;45% fat) and simultaneously trained with END and HIIT for 10 weeks; (2) Treatment (EX2): after 10 weeks of dietary intervention, another cohort of 10 week-old mice were trained by both programmes for 10 weeks.

Results

In EX1, END and HIIT decreased low-molecular weight adiponectin (∼0.5-fold; p < 0.05) and increased GLUT4 levels (∼2-fold; p < .05). In EX2, HFD significantly decreased high-molecular weight adiponectin (∼0.7-fold; p < .05), and END reversed this change.

Discussion and conclusion: HFD and exercise influence heart adiponectin isoforms and therefore might impact cardiomyocyte metabolism.

Keywords:

• Obesity
• exercise
• heart
• adiponectin
• GLUT4

Disclosure statement

The authors declare that they have no conflict of interest.

Additional information

Funding

S. M. -H. is supported by a National Commission for Scientific and Technological Research (CONICYT) Scholarship (Resolución Exenta #2185/2015). C. S. T. is supported by a National Health and Medical Research Council (NHMRC) Early Career Fellowship (#1037275). This work was supported by the Royal Prince Alfred Hospital Endocrinology Dept Trust Fund.