Reverse expression pattern of sirtuin-1 and histone deacetylase-9 in coronary artery disease

Abstract

Background

SIRT1 and HDAC 9 genes are related to inflammation and may contribute to the pathogenesis of coronary artery disease (CAD). We aimed to evaluate the expression level, methylation profile and polymorphisms of these genes in CAD patients.

Methods

In this study, 50 CAD patients and 50 healthy individuals were recruited. The expression level change was evaluated using the TaqMan Real-Time PCR method. The methylation of genes promoter and genotyping of polymorphisms were evaluated by the HRM.

Results

The expression level of SIRT1 was reduced while the HDAC9 expression level showed a significant elevation (p < .001). The SIRT1 gene promoter was hypomethylated and the HDAC9 gene promoter was hypermethylated in CAD patients. Also, CG + GG genotype in SIRT1 and both genotypes in the HDAC9 gene were associated with expression change.

Conclusions

SIRT1 and HDAC9 genes, expression changes can be suggested as a potential biomarker for CAD detection.

Keywords:

• Coronary artery disease
• HDAC9
• methylation
• polymorphism
• SIRT1

Acknowledgements

The authors would like to announce their appreciation to all patients who have achieved this study. This research was supported by Shahid Beheshti University of Medical Sciences. (grant no.10787).

Human subjects/informed consent statement

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study.

Disclosure statement

The authors declare that they do not have a conflict of interest.