Transcriptome profiling of transgenic tobacco (Nicotiana tabacum cv. xanthi nc) expressing CchGLP gene from Capsicum chinense Jacq. reveals gene expression associated with stress tolerance

ABSTRACT

Plants require effective sensing and responses to environmental stimuli, for example in the case of biotic and abiotic stresses. Reactive oxygen species (ROS) are well-recognised key signal molecules for the stress response. In previous work in our group, several transgenic tobacco lines were generated constitutively expressing a germin-like gene from Capsicum chinense (CchGLP), producing higher endogen levels of hydrogen peroxide that correlated with tolerance to geminivirus infections and drought stress. Differential transcriptomic analysis between transgenic tobacco lines of low (L1) and high (L8) CchGLP gene expression showed key changes in differential profiles related to the crosstalk between basal metabolism, biochemical and stress-related pathways that might explain the observed tolerant phenotype. Our results suggested that hydrogen peroxide levels modified transcriptomic pattern allowing tolerance in these tobaccos towards biotic and abiotic stresses. Constitutive production of endogen hydrogen peroxide in certain levels within the plants might allow plant protection for future adversely scenarios in global farming.

KEYWORDS:

• Biotic stress
• abiotic stress
• hydrogen peroxide
• tolerance
• elicitation

View correction statement:

Erratum

Acknowledgements

This research was partially supported by CONACyT (FORDECYT 193512) and Ciencia Básica SEP-CONACyT (178429). G.C-M. also acknowledges to CONACyT for grant support during the investigation.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

FORDECYT-CONACYT, México (Project number 193512) and Ciencia Básica SEP-CONACYT (Project number 178429) provided support for this research.