S-genotype characterisation of 10 Kernel-apricots native to China

ABSTRACT

The reproductive mechanism of Kernel-apricot involves Gametophytic self-incompatibility (GSI) which is under S-allele control. It encodes a polymorphic pistil ribonuclease (S-RNase), which is multiallelic and ideal markers for S-genotypes characterisation. In this study, six S-RNase primers were used to identify theS-genotypes of 10 Kernel-apricot, including three self-design primers and three universal primers. Ten S-alleles were identified in 10 Kernel-apricot through sequencing and comparing in NCBI. Moreover, some Kernel-apricot S-genotypes were confirmed by experiment of fruit-setting ratio and pollen tube growth. In conclusion, S-genotypes information of Kernel-apricot will be helpful for breeders and growers to plan compatible hybridised combination for ensuring effective pollination and high fruit-setting ratio.

KEYWORDS:

• Kernel-apricot
• S-genotypes
• self-incompatibility
• S-RNase

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This research was financially supported by Foundation of Henan Collaborative Innovation Center of Food Production and Safety [Grant No. FCIC201601] and Doctoral Research Startup Fund of Zhengzhou University of Light Industry [Grant No. 2015BSJJ037]