Effects of LED photobiomodulation therapy on the subcutaneous fatty tissue of obese individuals – histological and immunohistochemical analysis

ABSTRACT

Photobiomodulation therapy (PBMT) has become an adjuvant therapeutic possibility in body remodeling procedures. Given this scenario, this study was proposed with the aim of evaluating the effects of PBMT to Light Emitting Diode (LED) associating the red (630 nm) and infrared (850 nm) wavelengths in the subcutaneous fatty tissue. This controlled study of comparative intervention that evaluated a sample of subcutaneous fatty tissue from women with grade II obesity. The participants received the LED PBMT treatment with associated red and infrared wavelengths sequentially on the left side of the abdomen and the right side was considered as control, with the collection of biological material performed at the time of bariatric surgery. For histological and immunohistochemical evaluation, Caspase 3, Cleaved Caspase 3, CD68+, HSL and adipophilin markers were used. The participants showed positivity in the expression of Caspase 3 and Cleaved Caspase (p < .0001), CD68+ macrophages (p < .0001), HSL (p < .0001) and adipophilin (p < .0013) in the intervention sample when compared to the control. PBMT and LED associating red and infrared wavelengths were able to promote autophagic lipolysis induced by adipocyte cell apoptosis in the subcutaneous tissue of obese individuals.

KEYWORDS:

• Photobiomodulation
• LED
• noninvasive
• obesity
• lipolysis
• apoptosis

Contributions of all authors

PhD. Débora A. Oliveira Modena, data collection and analysis, writing of the article.

PhD Ciro Dantas Soares, data analysis, writing of the article.

PhD. Cintia Cristina Santi Martignago and Pt Stephani de Almeida, contributions in the writing of the article.

PhD. Everton Cazzo data collection, joint supervision of the research and contributions in the writing of the article

PhD. Elinton Adami Chaim, data collection, supervision of the research and contributions in the writing of the article.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

This study that has been performed in accordance with the 1964 Declaration of Helsinki and was approved by the Institutional Ethics Committee of the University of Campinas (Unicamp) under the recommendation number 2.281.487 and registered in clinical trials n° NCT05012514.

Additional information

Funding

This study did not receive any funding.