Variants of HSD11B2 gene in hypertensive disorders of pregnancy

Abstract

Introduction: One of the hypotheses concerning the etiology of gestational hypertension (GH) and pre-eclampsia (PE) assumes that they develop as a result of placenta malfunctioning at the early stage of pregnancy. Placental dysfunction is also associated with the decreased activity of 11β-hydroxysteroid dehydrogenase 2 (11β-HSD2), which in normal pregnancy protects the fetus from the excess of maternal cortisol.

Objective: The aim of the study was to analyze the sequence of HSD11B2, a gene that encodes 11β-HSD2, searching for mutations and haplotypes associated with the increased risk of GH or PE. Those may serve as potential genetic markers of GH and PE.

Methods: The study was performed in case-control structure and included pregnant women (in third trimester) diagnosed with: GH, PE or being normotensive (control group). The research comprised DNA sequencing of HSD11B2, followed by restriction analysis (PCR-RFLP). The linkage disequilibrium analysis and haplotype-based case-control analysis were performed.

Results: Six sequence variations were observed. Four mutations were indicated in the coding region of HSD11B2 and the other two in 3′-UTR. Two SNPs: c.468C > A and c.534G > A were found to be in total disequilibrium.

Conclusions: High variability in HSD11B2 sequence was indicated in the study population, but the relevance of observed SNPs to GH or PE development was not confirmed.

Keywords:

• Gestational hypertension
• pre-eclampsia
• glucocorticoid
• cortisol
• 11β-hydroxysteroid dehydrogenase type 2

Declaration of interest

The work was supported by the National Science Center (grant 2012/05/B/NZ7/02532) in Poland. The authors report no conflicts of interest.

Supplementary material available online

Supplementary Tables 1, 2 and 3