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Original Articles

Functional regulation of human trophoblast cells by CSE1L

, , , , , & show all
Pages 1598-1605 | Received 08 Jan 2019, Accepted 05 Jul 2019, Published online: 08 Aug 2019
 

Abstract

Objectives

To investigate the effects of the human chromosomal segregation 1-like (CSE1L) gene on the biological behaviour of human placental cells.

Study design

The CSE1L gene plays important roles in chromosome segregation during mitosis, cell proliferation and apoptosis. We used MTT, Cellomics, cell colony formation and flow cytometry assays in this study.

Results

CSE1L mRNA was significantly increased in human placental cells (JAR) and decreased when CSE1L was knocked down. Lentiviral CSE1L knockdown reduced cell proliferation (p < .05) and colony formation (p = .00) and induced apoptosis (p < .000). CSE1L knockdown inhibited the G0/G1 phase (p = .00) and increased the G2/M phase (p = .00) of the cell cycle, but no significant change in the S phase was observed (p = .158).

Conclusion

The CSE1L protein is expressed in human placental carcinoma cells, and its knockdown altered biological behaviours, implying that the CSE1L gene may affect human placental formation and even foetal development.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by grants of the National Natural Science Foundation of China, grant Nos. [81571461, 81873842, 81741038, 81801473, 81100447], Special Project Fund of Taishan Scholars, the Natural Science Foundation of Shandong Province, grant No. [ZR2016HM10]; and the Jinan Science and Technology Plan, grant No. [201602161]. Funding was provided by the two corresponding authors. They were responsible for the conception and design of the study.

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