Abstract
A new steroidal saponin was isolated from the leaves of Agave attenuata. Its structure was established as (3β,5β,25S)-spirostan-3-yl O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranosyl-(1→2)-O-[β-d-glucopyranosyl-(1→3)]-β-d-glucopyranosyl-(1→4)-β-d-galactopyranoside. The structural identification was performed using detailed analyses of 1H- and 13C-NMR spectra including 2D NMR spectroscopic techniques (COSY, HETCOR, and COLOC) and chemical conversions. The hemolytic activity of the steroidal saponin was evaluated using an in vitro assay.
Acknowledgements
The authors thank CNPq, FAPERJ, and FUJB for financial support.