Abstract
A new steroidal saponin was isolated from the leaves of Agave shrevei. Its structure was established as 3-[O-β-D-glucopyranosyl-(1→2)-O-[O-β-D-glucopyranosyl-(1→4)-O-[O-β-D-glucopyranosyl-(1→6)]-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl)-oxy]-(3β,5α,25R)-spirostane. The structural identification was performed using detailed analyses of 1H- and 13C-NMR spectra including 2D NMR spectroscopic techniques (COSY, HETCOR, HMBC, and HMQC) and chemical conversions. The haemolytic activity of the steroidal saponin was evaluated using an in vitro assay.
Acknowledgement
This work was financially supported by CNPq, FAPERJ, CAPES, and FUJB.